School of Science Department of Chemistry 3 Single Molecule Manipulation and Characterization of Biomolecules Supervisor: HUANG Jinqing / CHEM Student: LEE Dong Heon / CHEM Course: UROP1100, Summer The single-molecule study opens a new window to investigate molecular structures and their chemical properties. In this project, we focused on DNA stretching as it is widely used as a handle for optical tweezer experiments. The short and long DNAs are extended via optical tweezer then, their concordance rate and fitting parameters are derived. The results interpretation confirmed the current drawbacks of singlemolecule experiments. The short and long DNA experiments exhibited that the sample molecule can interfere with measurements, and utilized extensible WLC may be deficient in modeling the phenomenon. Further, by comparing the data of short and long DNA, we found that the stretching geometry or length of the molecule can also contribute to the difference in the result. Medicinal Chemistry on Novel Type I1/2 ALK Inhibitors for Combating Drug-resistant Mutants Supervisor: HUANG Yong / CHEM Student: MA Wing Yin Timothy / CHEM Course: UROP1100, Fall Anaplastic Lymphoma Kinase is a tyrosine kinase which is expressed in different types of cancer tumor. In non-small cell lung cancer (NSCLC), ALK gene loci on chromosome 2 has caused rearrangement in about 5% of NSCLC tumors. Detecting ALK gene and using suitable therapy is critical in treatment of lung cancer. Anaplastic Lymphoma Kinase (ALK) inhibition is a therapy targeting the ALK-positive tumors and preventing the expressing of this oncogene. This project is aimed to synthesis an ALK inhibitor molecule. Medicinal Chemistry on Novel Type I1/2 ALK Inhibitors for Combating Drug-resistant Mutants Supervisor: HUANG Yong / CHEM Student: WONG Tsz Wang / CHEM Course: UROP2100, Fall It is proposed that the meta, para selective borylation of aromatic esters and amide can be controlled using Ir-catalysis with different ligand in various length, shape and directing group. In the previous project, we have design 4 ligands and 2 of them have been synthesized. In this project, the remaining ligands are synthesized using optimized condition and the Selective CH Remote Borylation of Benzoxazole is studied using quaterpyridine ligand, L4. Nuclear Magnetic Resonance (NMR) Spectroscopy, Gas Chromatography/ Mass Spectrometer (GC/MS) are using to confirm the quality, quantity, and purity of the synthesized compounds.