UROP Proceedings 2020-21

School of Science Division of Life Science 12 Division of Life Science Studying Novel Cell Cycle Signalling Pathways in Yeast Cells Supervisor: BANFIELD David Karl / LIFS Student: SINCE Alec Victor / BIBU Course: UROP1100, Summer The purpose of this study is to investigate the stress-induced ectopic activation of the Spindle Assembly Checkpoint (SAC). We investigate the kinases of the cell wall integrity (CWI) pathway and determine whether they are involved in SAC activation. One of these kinases is Pkh1p and the deletion of this kinase seems to delay SAC activation. We came to this conclusion by using Mad2p-mNeon fluorescence microscopy imaging and calculated the proportion of cells that form Mad2p kinetochore foci. Further studies are required to determine whether the deletion of Pkh1p has a direct or indirect effect on delaying SAC activation or whether the Pkh2p homolog of Pkh1p can replace its function in its absence. Molecular Regulation of Skeletal Muscle Stem Cell Quiescence and Activation Supervisor: CHEUNG Tom / LIFS Student: LI Chun Wa / BCB Course: UROP1100, Fall Muscle satellite cells (MuSCs) perform a crucial role in skeletal muscle regeneration after injury. In this UROP 1100, emphasis is put on analysis on differential expression of these cells in post injury. Using the Galaxy platform, RNA-seq was performed using the Tuxedo pathway and Gene Set Enrichment Analysis (GSEA) using EGEA, which identified 7174 differentially expressed genes (DEGs) in 20367 loci. Via EGSEA, it was detected activation in molecular pathways for proliferation and differentiation such as E2F-dependent cell cycle transition, G2M checkpoint, and mTORC1 signalling. These data suggested that post-injured satellite cells may exit quiescent state and carry out proliferation after injury, possibly for regeneration of damaged skeletal muscle cells. Construction of a Signal Transduction Pathway Reporter Indicator for Monitoring Signaling Strength Supervisor: CHOW King Lau / LIFS Student: YUEN Yan Yi Macy / BIOT Course: UROP1100, Spring This project will manipulate the genetic model system, Caenorhabditis elegans, to recruit one of the novel gene components regulated by the transforming growth factor-β, DBL-1, as an indirect indicator of the BMP signaling strength. Sma-6, the target gene for the project, is the type I receptor for DBL-1 ligands in the BMP pathway and is transcriptionally regulated by the dbl-1 signaling. Thus, the gene expression of sma-6 can reflect the intensity of the BMP pathway. This experiment will develop the sma-6 transcription reporter responding to the dbl-1 signaling and amplify the readout of signal strength with the use of several reporters.

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