UROP Proceedings 2020-21

School of Science Division of Life Science 20 Receptor Based Drug Development from Chinese Herbal Medicine Supervisor: LI Ning / LIFS Co- supervisor: XUE Hong / LIFS Student: LEE Jiho / BIOT CHAN Teresa / BIBU Course: UROP1100, Spring UROP2100, Spring Wogonin, a member of flavone, is academically recognized for several therapeutic potentials. In particular, its anxiolytic activity by its affinity with the benzodiazepine (BZD) binding site on the γ-aminobutyric acidA (GABAA) receptor was recently discovered, making wogonin a candidate for relieving schizophrenic symptoms with fewer side effects. Extraction methods of this compound were extensively discussed in the literature, yet few were suitable for the industrial process scale-up. Dispersed Mobile-phase Countercurrent Chromatography (DMCC) provides continuous high-resolution separation for various compound types, with the potential to be applied to a large-scale wogonin purification. In this report, the extraction efficacy of wogonin from Scutellaria baicalensis Georgi with the DMCC method is to be discussed and evaluated. Receptor Based Drug Development from Chinese Herbal Medicine Supervisor: LI Ning / LIFS Co- supervisor: XUE Hong / LIFS Student: NG Chi Kit / BIBU Course: UROP1100, Spring Cancer, identified as a human complex disease by a combination of multiple gene defect and environmental factors, are regarded as one of the diseases with high fatality rate. To elucidate the genetic causes of the arise of cancer for an early analysis purpose, inter-Alu PCR sequencing is adopted to amplify the DNA sample concentration to a large extent, which can be identify the genomic sequence with a significant observation. Hence, through comparing the sequence among lung cancer patients and controls by a series of procedures, specifically BioRuptor fragmentation, gel fragment separation, end repairing, adenylation, indexation and finally next-generation sequencing, the DNA library can be constructed, and the single nucleotide variations can be identified as the fundamental for further research. Receptor Based Drug Development from Chinese Herbal Medicine Supervisor: LI Ning / LIFS Co- supervisor: XUE Hong / LIFS Student: WONG Hiu Man / BCB Course: UROP1100, Spring Alzheimer’s disease (AD) is one of the deep-rooted problems for the aging population due to the lack of potent drugs for complete treatment. To assist early diagnosis, it is of utmost importance to examine possible causes of this disease. It has been suggested Schizophrenia (SZ) could lead to higher risk of developing AD at later stages. Although recent studies have been extensively conducted to examine morphological and clinical similarities and differences between two diseases, there is no comprehensive research to analyse and compare the results of the behavioural tests and biochemical assays. This report will demonstrate the experimental results of behavioural tests on GABAA β2 subunit knockout mice to evaluate the effect of SZ on AD.

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