UROP Proceedings 2020-21

School of Science Division of Life Science 22 Anticancer Drug Resistant Mechanisms Supervisor: LIANG Chun / LIFS Student: CHIU Yui Hei / BIOT Course: UROP1100, Fall M2 is a potential anti-cancer drug candidate studied in Dr Liang Chun’s laboratory. The drug candidate shows its exceptional potential and specificity in inhibiting the growth of cancer cells, particularly Hela cells. However, the long-term use of the drug exhibits a critical problem– Drug resistance. Previously, a resistance population- R8 was studied in depth through different experiments, confirming its identity and characteristics. This progress report devotes itself into studying a higher resistance population- R10, a cell line developed from Hela Cell line which has the highest drug resistance among the populations in our lab. We found out that R10 population, like R8, has a very distinctive morphological and chemically compared to the Hela cell line, we believe that the differences are responsible for the even more aggressive and invasive behaviour. On top of that, we isolated R10 single cell clones and characterized for M2 resistance. We found out that R10 clones display higher survivability and recoverability in the presence of drug compared to the parental Hela cell line. Anticancer Drug Resistant Mechanisms Supervisor: LIANG Chun / LIFS Student: HO Phei Ting / BIOT Course: UROP1100, Fall The purpose of this report is to further evaluate the properties of Chinese herbs on Insulin Resistant (IR) HepG2 cells in-vitro. The main focus is to decipher Chinese herbs that can ameliorate the effect on diabetic Type II patients by comparing the glucose uptake assay on different incubation time. The herbs used in this research are Momordica charantia, Pericarpium granati and Radix astragali. Different experimental procedures such as cell subculture, WST1 assay, Glucose Uptake Assay and Western Blot were conducted to study the underlying effect on IR cells and concluding with most significant herb using the experimental results gathered. Furthermore, the analysis of specific proteins that play specific roles in glucose uptake were further studied (GLUT-2 and IRS-1 against a reference protein: β-Actin).