UROP Proceedings 2020-21

School of Science Division of Life Science 23 Anticancer Drug Resistant Mechanisms Supervisor: LIANG Chun / LIFS Student: GUO Yuchen / BCB CHIU Yui Hei / BIOT Course: UROP1100, Spring UROP2100, Spring M2 is a potential anti-cancer drug candidate studied in Dr Liang Chun’s laboratory. The drug candidate shows its exceptional potential and specificity in inhibiting the growth of cancer cells, particularly Hela cells. However, the long-term use of the drug exhibits a critical issue– Drug resistance. Previously, 2-8, 4-5 and 56 clones from the R10 population (Highest drug resistance HeLa population available in our laboratory) were studied in depth through various assays, confirming their morphology. This project devoted itself into studying another two clones; 2-9, 4-4 in comparison with the HeLa population by conducting various assays. We found that 2-9 and 4-4 exhibited similar trends as the previous R10 clones with slower growth, higher survivability after drug treatment and smaller in size. Unlike the previous clones, 2-9 and 4-4 exhibited different behaviours implying different degree of resistance to the M2 drug. Anticancer Drug Resistant Mechanisms Supervisor: LIANG Chun / LIFS Student: WOO Ho Wun Howard / BIOT Course: UROP1000, Summer The chemical changes in the cell environment alter the mechanical cues that lead to variations in biochemical signaling and inherent process such as mitosis. In the current study, we explored the process of Daughter Number Variations (DNVs) in M2 drug-resistant single cell line clones (SCCs) relative to parental HeLa. The three SCCs derived from the R10 resistant population were selected to characterize for resistance mechanism and DNV events via live-cell microscopy for 24 hours. The SCCs observed resulted in stress cell division: DNVs events (from 3 to 5 daughter cells), delayed mitosis, uneven prolonged cell size, and cell death induction. In resistant clones, the multi-daughter division was more common and exhibited amplified cell size and extended division time. Cell fusion after the division was also seen in SCCs. However, further experiments are required to understand the mechanism at the molecular level.

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