UROP Proceedings 2020-21

School of Science Division of Life Science 24 Chinese Herbal Medicine for General Health Supervisor: LIANG Chun / LIFS Student: LAU Siu Wa / BCB Course: UROP1000, Summer Traditional Chinese medicine (TCM) have been widely applied in the Chinese community for thousands of years. It is also known as the oldest system of medicine in the world. In the old practice, practitioners prescribed drug based on their experience and literatures written by the predecessor. However, the performance of these herbal medicines is not well elucidated. So, this project aims to characterize herbs that can treat type 2 diabetes. We would like to investigate the performance of TCM in curing the disease. We have chosen Wolfiporia extensa (茯苓), Glycyrrhiza uralensis (甘草) and Astragalus membranaceus (黃芪) as the samples. It turned out all of them could facilitate the glucose uptake of insulin resistant cells (HepG2), hence lowering the glucose level in the culture medium. However, the difference among various concentrations was not distinct due to the uneven distribution of cells. We have also studied the toxicity of the TCM. All samples showed less toxicity than metformin, which is the first-line medication for the treatment of type 2 diabetes. Some of them could even promote the viability of cells. Chinese Herbal Medicine for General Health Supervisor: LIANG Chun / LIFS Student: WU Zehaoyu / BCB Course: UROP1100, Summer Dyslipidaemia, especially hyperlipidaemia has become known for its threat to the cardiovascular system in the recent years and various treatments are designed for this disorder. It is noticed that traditional Chinese medicine (TCM) also has the potential to treat this disorder but with a lower side effect. This study utilized HepG2 cell line to establish a hyperlipidaemia cell model to examine the preventing and relieving efficacy on hyperlipidaemia of two TCM single herbs, ChenPi and HuangQi by measuring the level of protein content, triglyceride (TG) and total cholesterol (TC) and the visualization of ORO assay, which significantly demonstrates the lipid controlling effect of two tested TCM components. DNA Replication-Initiation Proteins in Budding Yeast Supervisor: LIANG Chun / LIFS Student: SUN Chang / SSCI Course: UROP1100, Fall Noc3p is a key protein required for DNA replication licensing. It is required for the assembly of various other DNA replication-initiation proteins (DRIPs) for the initiation DNA replication. Based on previous studies in our lab, it is known that Noc3p and ORC subunits self-interact and form dimers during pre-RC formation. However, the mechanism of such self-interaction remains unclear. To further elucidate the mechanism of Noc3p self-interaction, in this study, NOC3-FRB cells were used to deplete free Noc3p from the yeast cell nucleus and determine the impact of depletion on Orc2p self-interaction.