UROP Proceedings 2020-21

School of Science Division of Life Science 25 DNA Replication-Initiation Proteins in Budding Yeast Supervisor: LIANG Chun / LIFS Student: CHENG HE Emily Isabel / BCB GAO Xuanting Tina / BCB Course: UROP1000, Summer UROP1000, Summer Nucleolar complex associated protein 3 (NOC3) plays a crucial role in DNA replication initiation. Fully elucidating the role and function of this protein will enhance our understanding of DNA replication and lay the foundation for further application in cancerrelated treatment. It is hypothesized that phosphorylation plays an essential part in the dimerization of NOC3, Origin Recognition Complex (ORC) dimerization and preRC formation. To verify this hypothesis, yeast cells expressing endogenous NOC3-HA protein were used. Chromatin Binding Assays and immunoblot analysis were carried out to examine the phosphorylation status of Noc3p. The result of the immunoblotting indicates that Noc3p undergoes dephosphorylation in G1 phase and phosphorylation in S and M phases, which confirms the project hypothesis. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: CHAN Wing Lam / BIBU Course: UROP1100, Fall Transposons are sequences that can either move or jump within a genome. It plays a crucial role in human evolution by rising new genes through the process of domestication. Meanwhile, transposon insertion potentially disrupts the normal function of genes. Recently, some research groups discover that transposons may contribute to the formation of tumors and thus results in colorectal cancer. In view of the role of transposons, Alu-scan sequencing was performed in this study to understand the linkage of transposons and the causes of human colorectal cancer. By performing the whole-genome sequencing technique, it is aimed to study the distribution of Alu elements in genomes of patients with colorectal cancer and healthy people. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: IU Wing Ting / BIBU Course: UROP2100, Fall There are many proposed genetic causes for schizophrenia, a complex human disease affecting the brain. In which, the Gabrb2-origin has been studied extensively recently. Gabrb2 is the gene coding for the β2 subunit of the GABAA receptor and studies found that the Gabrb2-knockout mice model displayed schizophrenia-like behavior, which can be a potential model to study the genetic cause of schizophrenia. This project aims to study the neuroinflammation status in different ages of Gabrb2-knockout mice by measuring the amount of pro-inflammatory cytokines: interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), by Enzyme-Linked Immunosorbent Assay (ELISA). With the data obtained, the start of neuroinflammation can be known, and the most suitable time for drug administration can be determined.

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