UROP Proceedings 2020-21

School of Science Division of Life Science 26 Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: KAN Chloe / BTGBM Course: UROP3100, Fall The GABAA receptors are pentameric protein channels that mediate neural inhibition in the central nervous system, hence they are crucial for the balance of neuronal activity. Due to their function, the receptors play a key role in neuropsychiatric disorders such as schizophrenia and epilepsy. Many psychotropic drugs target the GABAA receptors, so to better design effective drugs, the structure of GABAA receptors and the key residues for binding should be clearly identified. Hence, this study aims to investigate the structure of the key fragments of the α1, β2, and γ2 subunits, as well as the native isoform of GABAA receptors. This will help obtain a higher resolution atomic structure of the receptor. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: KAN Chloe / BTGBM Course: UROP4100, Spring The GABAA receptors are channel proteins that contribute to the inhibition of neurotransmitters in the central nervous system, and mediate neuronal balance. The receptors are crucial in many neuropsychiatric disorders, including schizophrenia. Drugs such as benzodiazepines also target these receptors, so the structure of the receptors are key to identifying and designing better medicine that is more effective for treating disorders. Therefore, this study aims to explore and visualize the structure of the GABAA receptors and investigate the effects of the three predominant subunits on drug binding. Hopefully, this study will lead to a deeper understanding of the atomic structure of GABAA receptors and aid in the development of neuropsychiatric drugs. Human Complex Disease Genomics and Bioinformatics Supervisor: LIANG Chun / LIFS Co-supervisor: XUE Hong / LIFS Student: HUI Cheuk Him / BIBU Course: UROP1100, Summer Lung cancer is the leading cause of cancer deaths worldwide. With the goal of improving cancer prognosis and treatment, Next Generation Sequencing (NGS) was adopted to discover the genetic causes of this disease. Prior to NGS, DNA Libraries of nine samples were prepared. Procedures include DNA extraction, Alu PCR, fragmentation, end repairing, adenylation, adapter ligation, and KOD PCR. Five to eight cycles of ultrasonic shearing succeeded in creating DNA fragments of sizes within 300 and 400bp. Five purification rounds resulted in 7.5 % recovery of desired DNA fragments. The largest decline in DNA amount occurred after KOD PCR. 99 to 213 ng of DNA remained per sample.

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