UROP Proceedings 2020-21

School of Science Division of Life Science 27 Potential Cancer Drug Identification and Characterization Supervisor: LIANG Chun / LIFS Student: KWON Daye / SSCI Course: UROP1100, Fall Studies have indicated the potentials of TCM (Traditional Chinese Medicines) for treatment of insulin resistance. In the present experiment, the efficiencies in the uptake of glucose of three popular TCMs— Chinese Cinnamon, Polygonatum odoratum, Radix bardanae were compared to that of a commonly known diabetes drug, Metformin. Insulin-resistant HepG2 cells were treated with each drug solutions and were screened with addition of glucose assay kit. Absorbance at 490 nm of the treated HepG2 were measured and a glucose standard curve was plotted to obtain the absolute glucose uptake quantity for comparison. Although metformin showed the highest uptake of glucose, the results also suggested high efficiencies of glucose uptake stimulation and potentials of Polygonatum odoratum and Radix bardanae. Potential Cancer Drug Identification and Characterization Supervisor: LIANG Chun / LIFS Student: KWOK Nicholas Tze Yin / BTGBM Course: UROP1100, Spring With the proposal of minichromosome maintenance (MCM) proteins as cancer hallmarks, especially on enhanced proliferation and regulation of replicative stress, we aimed at discovering anticancer drugs via analyzing the drug efficacy towards MCM inhibition. AC5, a small molecule obtained from a preliminary screen of a chemical library, shows efficacy against MCM chromatin binding in cancer cell lines, i.e. HeLa and MCF7, and hence cytotoxic effect. It also shows selectivity, causing less damage in normal cell line MCF10A and significantly better recovery than cancer cell lines. This study shows the potential of AC5 as a novel anticancer drug candidate for later preclinical research. Potential Cancer Drug Identification and Characterization Supervisor: LIANG Chun / LIFS Student: WONG Lok Lam / BIBU Course: UROP1100, Spring Chinese herbal drugs have long been recognized for the focus on holistic physical health condition and thus have few side effects. However, it is more common to use Western oral drugs to treat diabetes nowadays. With the consideration of the increasing incidence of diabetes, there is a need to discover more efficient drugs. Chinese herbal drugs may have advantages over the traditional medical approach, in terms of efficacy and side effects. In this experiment, the glucose uptake level of insulin resistance cells is investigated after herbal drugs treatment, so as to understand the efficacy of the drugs. WST-1 is also used to test the cell viability to ensure that the drugs are not toxic to cells.

RkJQdWJsaXNoZXIy NDk5Njg=