UROP Proceedings 2020-21

School of Science Division of Life Science 28 Potential Cancer Drug Identification and Characterization Supervisor: LIANG Chun / LIFS Student: WU Zehaoyu / BCB Course: UROP1100, Spring Dyslipidaemia is one of the most significant factors that leads to the cardiovascular diseases (CVD) around the world. In China, traditional Chinese medicine (TCM) has been applied long to prevent and treat this disorder in favour of its lower adverse effects. This study utilized HepG2 cell line to establish a hyperlipidaemia cell model to examine the efficacy of two TCM single herbs, ChenPi and HuangQi through WST-1 and ORO assay. Comparing the outcome with the classic statin and fibrate medications, the efficacies of TCM are around a similar level. The potential of two tested components on lipid controlling is thus well exhibited. Potential Cancer Drug Identification and Characterization Supervisor: LIANG Chun / LIFS Student: LEE Tung Yeung / SSCI WU Chak Lam Jerome / BCB Course: UROP1000, Summer UROP1000, Summer Minichromosome Maintenance (MCM) complex is crucial for DNA replication initiation and elongation in eukaryotes and was shown to increase cell proliferation through enhancing DNA replication rate. Therefore, drugs inhibiting the assembly of MCM complex is a valid approach in curing human cancer. In this project, RPE-1 cells stably expressing MCM2- EGFP is generated and characterized aiming to set up a platform for fast and convenient screening of potential anticancer drugs. Moreover, suitable conditions for drug screening using the platform have also been tested to enable better measurement of MCM-inhibition ability of candidate drugs in preliminary studies. Molecular Regulation of Axon Regeneration Supervisor: LIU Kai / LIFS Student: CAO Jiaming / BCB Course: UROP4100, Fall The human nervous system consists of central nervous system (CNS) and peripheral nervous system (PNS). In adult mammals, PNS axons can regenerate after injury while CNS axons do not have the ability to regenerate after lesion. Understanding CNS axon regeneration is an important step that in the future could lead to treatments for spinal cord injury, stroke, ALS and also other neurodegenerative disorders. There are both extrinsic factors and intrinsic factors for the regeneration failure of adult mammalian CNS axons, and the molecular mechanisms behind axon regeneration is still under research currently. To research the molecular regulation, we manipulate genes in vivo or in vitro using mouse model and observe the regeneration extent.