UROP Proceedings 2020-21

School of Science Division of Life Science 34 Standardization of Traditional Chinese Medicines Supervisor: TSIM Karl Wah Keung / LIFS Student: ABDRASSILOVA Kamila / BIBU Course: UROP1100, Spring Ameliorating skin conditions, such as atopic dermatitis (AD) and psoriasis, are linked to chronic inflammation. Edible bird’s nest (EBN) has demonstrated anti-inflammatory activity but the mechanisms of action are still investigated. Here, we review signalling pathways and proteins during inflammation that potentially could be targeted by EBN’s anti-inflammatory action. Moreover, the cellular synthesis of filaggrin, a protein being downregulated in AD, has been shown to be regulated by GATA3, a transcriptional factor. Here, we demonstrate results from RT-PCR, where EBN extract, EBN digest and N-acetylneuraminic acid (NANA) lead to significant increase in expression of GATA3 mRNA. Additionally, we suggest that the action of EBN having PPARα or PPARγ as modulator is worth to be investigated. Understanding the mechanism behind antiinflammatory properties of EBN can be applied to the development of topical creams in skin diseases. Standardization of Traditional Chinese Medicines Supervisor: TSIM Karl Wah Keung / LIFS Student: CHOI You Jin / BIOT Course: UROP1100, Spring Human skins, eyes, and hairs exist in a wide spectrum of colors and gradients due to the production of certain pigments known as melanin, which provide a defence mechanism against UV radiation and reactive oxygen species (ROS). Melanin pigments are produced and accumulated in melanosomes, a particular organelle within melanocytes, through the process called melanogenesis. A diversity of literature reviews demonstrates that melanosomes are being transported from melanocytes to a neighboring cell, named keratinocytes, to perform various functions, such as proliferation, differentiation, dendritogenesis, and melanogenesis. However, an abnormal increase in melanin production may induce medical problems, therefore demanding scientists are aiming to understand factors causing melanogenesis, in order to produce therapeutic solutions. Standardization of Traditional Chinese Medicines Supervisor: TSIM Karl Wah Keung / LIFS Student: WONG Tsz Yan / BIBU Course: UROP1100, Spring Cannabidiol (CBD) prevalent cannabis derivatives are increasingly popular and widely available on the market as replacement of THC, tobacco substitutes or therapeutics for various health conditions. The most two common ways to take CBD are oral and topical in which delivery methods include capsules, tinctures, balms, creams and more. In this project, CBD volatility in traditional Chinese medicine (TCM) essential oil is evaluated, which aims to test the feasibility of pulmonary administration in intaking CBD apart from traditional methods. This report devotes itself to utilizing High Performance Liquid Chromatography (HPLC) system coupled with Triple Quadrupole LC/MS to study the concentration of CBD in essential oil after a designated period of time, and the data could be analyzed to determine the best TCM essential oil in evaporating CBD during the administration.