UROP Proceedings 2020-21

School of Science Division of Life Science 40 G Protein-Coupled Receptors in Cell Signaling Supervisor: WONG Yung Hou / LIFS Student: CHOY Hiu Ching / BIBU Course: UROP1100, Spring Nm23 protein was the first metastasis suppressor being discovered. Nm23-H1/H2 exhibit multifunction with enzymatic activities, protein-protein interactions and DNA interactions. Studies have suggested that transcriptional targets of Nm23-H1/H2 include MMP2, EDG2, MYC and PDGF-A. PDGF-A, MMP2 and EDG2 are downregulated while MYC is upregulated by Nm23-H1/H2. This study demonstrates that Nm23-H1/H2 may indeed suppress MMP-2 transcription in 1321N1 cells and activate MYC transcription in MDA-MB-231 cells. Interestingly, Nm23- H1/H2 activated EDG2 transcription and suppressed MYC transcription in 1321N1 cells. However, the study failed to demonstrate PDGF-A gene suppression by Nm23-H1/H2. The results suggested that transcriptional regulations of cancer-associated genes may contribute to metastasis suppression function of Nm23-H1/H2, and the regulations may vary in different types of cancers. Bioinformatic Analysis of Single-Cell Genomic Data Supervisor: WU Angela Ruohao / LIFS Student: CHENANANPORN Pansit / BIEN Course: UROP1000, Summer This abstract summarizes the progress of sequencing reads using different assemblers. A sample assembly of the SARS-CoV-2 genome was performed using SPAdes. To replicate short reads produced by a sequencing machine, a complete assembly was fragmented into segments with sizes of 100 to 150 base pairs. Formatted into a FASTA file, these fragments were then inputted into SPAdes. The UCSC Blat Search Genome tool showed that the assembly was complete with a few identity mismatches. An assembly of a local sea urchin species was also performed. BUSCO was used to assess the genome by comparing its evolutionary similarity with an existing dataset (metazoa_odb10). However, there was a high percentage of duplicated orthologs. Thus, the next step, which is still currently worked on, is to remove overlaps in the assembly. Additionally, an assembly of the sea urchin data using SPAdes will be performed and compared with that of using Hifiasm.

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