School of Science Division of Life Science 41 Big Data: Bioinformatic Analysis of Single-Cell Genomic Data Supervisor: WU Angela Ruohao / LIFS Student: WU Ka Hei / GBUS Course: UROP1100, Summer As the global population continues to age, age-related neurodegenerative disorders are predicted to increase to 135 million cases worldwide by 2050, overtaking cancer as the second leading cause of death after cardiovascular disease (Gammon, 2014). In this study, we aim to create a singlenucleus RNA seq (snRNA-seq) library to facilitate the study of neurodegenerative disorders. We focus on analyzing the gene expression in three regions of the mouse brain – cortex, hippocampus, and cerebellum, which we will use at a later stage in this study to identify any aging-related molecular and cellular changes in the brain by comparing mice of 3-months vs. 17-months of age. This progress report will focus on featuring current annotated brain regions and account for the method used to derive these annotations. Genetic Studies on Stem Cell Regulation Supervisor: XIE Ting / LIFS Co-supervisor: TU Renjun / LIFS Student: LEE Man Ping / SSCI Course: UROP1000, Summer In the Drosophila ovary, germline stem cell (GSC) development is controlled both intrinsically and extrinsically. The niche, which provides special microenvironments for GSC development. RNA-binding proteins (RBPs) are critical in controlling gene expression through regulating RNA stability, decay, splicing or translation. However, the functions and mechanisms of RNA binding proteins in controlling germline stem cell development remain unclear. Therefore, identifying the RNA binding proteins essential for germline stem cells intrinsically and in the differentiation niche helps to understand how these genes control the development of GSC. By using genetic screening, we found that knocking down snRNP-U1-70K, clp or srrm1 in the differentiation niche with c587-Gal4 causes severe ovary development defects, differentiation defects. We also identified CG7903, pAbp, Imp and FNE are intrinsically required for GSC self-renewal. In the future studies, we will further how these RBPs control GSC self-renewal and differentiation molecularly.