School of Engineering Department of Chemical and Biological Engineering 86 Cathode Materials for Lithium-Ion Batteries Supervisor: SHAO Minhua / CBE Student: KIM Hyeongwoo / CENG Course: UROP1100, Spring Among many options, high voltage cathode materials are considered a vital element for the next-generation Li-ion battery. How to improve the cycling stability of LiNi0.8Co0.1Mn0.1O2 (NCM811) is a hot topic because of its outstanding capacity density, good rate performance but unfavorable cycling stability nature. The solid electrolyte interface (SEI) modification through electrolyte additives is one way of improving the performance of the cell, and fluoroethylene carbonate (FEC) is frequently used as a co-solvent. Cells assembled with NCM811 cathode, Li metal anode, and FEC-based electrolyte are subjected to charging and discharging cycles to observe the capacity retention after 100 cycles. A color-changing phenomenon related to FEC-based electrolytes is also discussed. Developing Genetically Encoded Molecular Tools for Controlled Intracellular Phase Separation Supervisor: SUN Fei / CBE Student: LO Shin Dawn / BIEN Course: UROP1100, Summer CarHc is part of a photoreceptor protein that can be split into two segments known as CarHcN and CarHcC, which contains the cobalamin-binding domain. Since CarHc is a split protein, it has the potential to be controlled by researchers via induction of its components to reassemble and even disassemble. This property can be applied to study proteins, control molecular function, and assemble protein architecture. In this study, the cobalamin-bindingcapacity and subsequent photosensitivity of CarHcC was investigated. It was found that CarHcC alone was indeed capable of binding with cobalamins cyanocobalamin (CNB12), adenosylcobalamin (AdoB12), and methylcobalamin (MeB12); the protein was even photosensitive when bound to AdoB12. This discovery confirms that the binding domain is located on the C-terminus split protein component. This discovery has implications for the possibility of genetic modification of CarHc – specifically, the CarHcN component – to tailor the protein for specific applications. Analyzing the Possible Water-Soluble Proteins for Deep-Tissue and Cancer-Targeting in Vivo Imaging Using a Photoacoustic Imaging System Supervisor: WONG Tsz Wai / CBE Student: CHENG Leong / CHEM Course: UROP1100, Summer To overcome the disadvantages associated with cancer-diagnosis methods commonly used today, including low molecular specificity from ultrasound imaging, highenergy radiation from X-rays, and gadoliniumdeposition nature of MRI, the photoacoustic imaging system is proposed. This modality allows for noninvasive and deep-tissue in vivo imaging through ultrasound waves generated by the thermoelastic expansion that is induced by light energy absorbed by tissues. However, in order to produce high resolution photoacoustic images, a highly specific contrast agent that satisfies several criteria is required; some of these critical criteria include aqueous stability, biocompatibility, and redshift. Thus, this report investigates several potential candidates for the contrast agent and selects the optimal one using the aforementioned criteria.