UROP Proceedings 2021-22

School of Science Division of Life Science 12 Molecular Regulation of Skeletal Muscle Stem Cell Quiescence and Activation Supervisor: CHEUNG Tom / LIFS Student: WONG Tsz Yan / BIBU Course: UROP1100, Summer Quiescence and activation of stem cells have been previously proven to be correlated to autophagy, which is the process of self-degradation of proteins. Utilizing muscle stem cells, or satellite cells isolated from adult skeletal muscle fromhindlimb of mice with fluorescence-activated cell sorter (FACS), the cell fate is examined from inhibition and promotion of autophagy. The autophagy granules, namely autophagosomes and autolysosomes are also evaluated with confocal microscope. As autophagy is closely related to ageing, the study of autophagy of satellite cell can provide insights for therapeutic development. Construction of a Signal Transduction Pathway Reporter Indicator for Monitoring Signaling Strength Supervisor: CHOW King Lau / LIFS Student: YUEN Yan Yi Macy / BIOT Course: UROP3100, Fall This project will manipulate the genetic model system, Caenorhabditis elegans, to recruit two of the gene components regulated by the transforming growth factor-β related ligand, DBL-1, as the indirect indicators of the dbl-1 signaling strength. sma-6, one of the target genes for the project, encodes the type I receptor for DBL-1 ligands and is positively regulated by the dbl-1 signaling. The second gene, gcy-28, is a receptortype guanylate cyclase and acts as a repression target in the BMP pathway. Thus, the gene expression of sma-6 and gcy-28 can reflect the intensity of the BMP transduction signal. This project will develop the sma6 and gcy-28 transcription reporters responding to the dbl-1 signaling and amplify the readout of signal strength through the manipulation of these two reporters in a variety of dbl-1 mutant strains. Monitoring the Norm of Human Morphology Supervisor: CHOW King Lau / LIFS Student: LUI Yuen Yee / SSCI Course: UROP1000, Summer The purpose of this paper is to test whether the body shape of modern human beings can also be described with parameter defined by the “Vitruvian man”. Quantitative and qualitative data were collected through the measurement of online photos. The data collected indicate that most of the statements described on the Vitruvian man are not correlated to the experimental observations. Distinct differences were found when comparing the results of two races and two sex groups. These distinct differences further act as several defining parameters to distinguishing two different races and two sex groups. This paper and experimental results can be used in investigating the biological correlation of distinct body proportion difference.