School of Science Division of Life Science 14 Analysis of Surface Delivery of Epidermal Growth Factor Receptors Supervisor: GUO Yusong / LIFS Student: HUNG Shing Hei / IRE Course: UROP1000, Summer The analysis of EGFR is highly related to understanding the development of cancer cells. The L858R mutation on EGFR is one of the most common mutations identified in lung cancer. T790M mutation on EGFR causes drug resistance of EGFR(L858R). This project is trying to introduce the further mutation T790M on EGFR(L858R) for further biological analysis. Analysis of Surface Delivery of Epidermal Growth Factor Receptors Supervisor: GUO Yusong / LIFS Student: LI Chun Wa / BCB Course: UROP1100, Fall UROP2100, Spring Mitochondria has been extensively studied since its discovery of the organelle in 1857 by physiologist Albert von Kolliker, unravelling many features of this bacterial descendent, from its fission and fusion machinery, to its interactions with other organelles via signaling or membrane contact sites. Mitochondrial-derived vesicles (MDVs) is an emerging field that is comparatively less studied yet exciting in terms of understanding mitochondrial regulations and their physiological relevance. MDVs are responsible for diverse cellular processes, in which its role in mitochondria quality control (MQC), peroxisome biogenesis and mitochondria antigen presentation (MitAP). The molecular machineries controlling cargo selection, budding and trafficking of these processes will be discussed in this review. Analysis of Surface Delivery of Epidermal Growth Factor Receptors Supervisor: GUO Yusong / LIFS Student: PARK Jihye / BIOT Course: UROP1100, Spring Sonic Hedgehog (Shh), one of the three Hedgehog (Hh) family members, is an important signalling molecule involved in many key developmental processes, such as embryonic patterning, cell differentiation, and organ development. The Shh protein undergoes two major changes in the endoplasmic reticulum (ER). It is first auto-cleaved into two parts, a C-terminal fragment (ShhC) and an N-terminal fragment (ShhN). ShhC is degraded, while ShhN is exported from the ER after lipid modification and delivered to the cell membrane to initiate signal transduction. Although the signal transduction pathway is well-understood, the complete mechanism behind Shh protein secretion is still undiscovered. Therefore, this paper examines the transport of the N-terminal fragment of Shh without the lipid modification (ShhN) in vitro to unravel the molecular mechanisms that direct the trafficking and secretion of Shh.