UROP Proceedings 2021-22

School of Science Division of Life Science 30 Study of Blood Cell Development Using Zebrafish Model Supervisor: WEN Zilong / LIFS Student: LI Zhuying / BCB Course: UROP2100, Fall UROP3100, Spring UROP4100, Summer γδ T cells are considered as a T cell subset that play an intermediate role between innate and adaptive immunity. They display T cell receptors (TCRs), but have distinct way of recognizing their target antigens. They do not recognize peptide antigens presented by antigen presenting cells, but directly respond to infection consequence, giving them more innate-like features. In zebrafish, γδ T cells mainly distribute in the skins, gills and intestines. chemokine (C-C motif) ligand 38, duplicate 6 (ccl38.6) is a gene exclusively exists in zebrafish and may be closely associated with γδ T cells. This report will introduce the progress of our project on γδ T cells via research on ccl38.6. Calcareous Dinoflagellates Supervisor: WONG Joseph Tin Yum / LIFS Student: MONG Sze Hang / BIOT Course: UROP1100, Fall Due to elevation of carbon dioxide concentration in the atmosphere, the pH value of seawater has been decreasing over the decades, which may facilitate the calcite dissolution and fluctuated the bicarbonate and carbonate content in seawater. Given the fact that carbonate ion is one of the building blocks of biomineralization of calcareous dinoflagellates, it is proposed that ocean acidification will affect the calcification and growth of calcareous dinoflagellates. Having established a fundamental knowledge regarding the various life cycle alterations of Thoracosphaera heimii. from the previous project, this report would like to investigate how increasing bicarbonate and carbonate ion will affect the morphology as well as the cell population of T. heimii. G Protein-coupled Receptors in Cell Signaling Supervisor: WONG Yung Hou / LIFS Student: LEE Tung Yeung / BCB Course: UROP1100, Spring UROP2100, Summer Guanine nucleotide-binding protein alpha-subunit-i (Gαi) is known to interact with adenylate cyclase (AC) and exert an inhibitory effect on the conversion from ATP into an important secondary messenger cAMP. However, the domain(s) contributing to its inhibitory effect is not completely understood. Together with the results obtained from the last semester, we generated 8 sets of Gαi1 mutants at the chi1 (residue 297-318) region aiming at mapping out all the residues that may interact with AC. The chi1 region, together with N256, K257, and W258 in Gαi1, had been shown to effectively reduce the inhibitory effect on AC. Moreover, we have confirmed the protein expression levels of the mutants generated for future functional studies.