School of Engineering Department of Chemical and Biological Engineering 71 Combinatorial Lipopeptides for Immunomodulation Supervisor: CHAU Ying / CBE Student: YAP Octavius Jessica / CENG Course: UROP1100, Spring UROP2100, Summer Macrophages are widely circulated innate immune cells that play a crucial role in progression and resolution of inflammation. In response to its local microenvironment, macrophages can polarize into M1 phenotype which has pro-inflammatory characteristic, and M2 phenotype which perform anti-inflammatory feature. However, macrophage polarization disorder can damage tissue during inflammatory diseases and promote development of tumor. Hence, regulating macrophage polarization in specific disease could be a promising therapeutic strategy. Various peptide sequences are known to be responsible in macrophage polarization. These peptide signals were conjugated on the surface of micro-sized hydrogel and were presented as solid bound signals. The effect of peptide bound signal and its peptide combination on macrophage polarization were examined. Combinatorial Lipopeptides for Immunomodulation Supervisor: CHAU Ying / CBE Student: LI Ho Wa / BIEN Course: UROP1100, Spring Macrophages host the ability to generate different phenotypes via multiple pathways and acts as an important regulator in the development of cancer and different metastasis stage. M1 macrophages promotes inflammatory response and anti-microbial activities while M2 group macrophages serves as pro-healing and anti-inflammatory activator. (Jayasingam, S. et al., 2020; Orecchioni, M. et al., 2019; Gordon, S., 2017) Modulators including peptides on hydrogel can induce diverse polarization. However, the exact mechanism behind M1/M2 polarization and the effect of external factors including the morphology of the hydrogel is still unknown. To understand and predict this complex relationship for immunomodulation, hydrogel presenting peptides with different combinations are generated and different machine learning algorithms are built to understand its complex relationship between different properties of peptide, hydrogel and macrophage polarization.